2000-2009

Main content

2009

 

An aspartate and a water molecule mediate efficient acid-base catalysis in a tailored antibody pocket 
Debler, E. W., Müller, R., Hilvert, D., and Wilson, I. A., Proc. Natl. Acad. Sci. U. S. A. 2009, 18539-18550

A novel noncovalent complex of chorismate mutase and DAHP synthase from Mycobacterium tuberculosis: protein purification, crystallization and X-ray diffraction analysis
Oekvist, M., Sasso, S., Roderer, K., Kast, P., Krengel, U., Acta Cryst. Section F 2009 1048-1052

Kinetic characterization of spiropyrans in aqueous media
Stafforst, T., and Hilvert, D. Chem. Commun. 2009, 287-288

A rationally designed aldolase foldamer
Müller, M. M., Windsor, M. A., Pomerantz, W. C., Gellman, S. H., and Hilvert, D., Angew. Chem., Int. Ed. Engl. 2009, 48, 922-925.

Probing the role of the proximal heme ligand in cytochrome P450cam by recombinant incorporation of selenocysteine
Aldag, C., Gromov, I. A., Garcia-Rubio, I., Von Koenig, K., Schlichting, I., Jaun, B., and Hilvert, D. 2009 , Proc. Natl. Acad. Sci. U. S. A. 106, 5481-5486.

Evolutionary Cycles for Pericyclic Reactions - Or Why We Keep Mutating Mutases
Roderer, K., Kast, P., Chimia 2009 313-317

 

2008

 

Relative tolerance of an enzymatic molten globule and its thermostable counterpart to point mutation
Woycechowsky, K. J., Choutko, A., Vamvaca, K., and Hilvert, D. 2008 , Biochemistry 47, 13489-13496.

Structural reorganization and preorganization in enzyme active sites: Comparisons of experimental and theoretically ideal active site geometries in the multistep serine esterase reaction cycle
Smith, A. J. T., Müller, R., Toscano, M. D., Kast, P., Hellinga, H. W., Hilvert, D., and Houk, K. N. 2008 , J. Am. Chem. Soc. 130, 15361-15373.

Synthesis of β-hydroxy-α-amino acids with a reengineered alanine racemase
Fesko, K., Giger, L., and Hilvert, D. Bioorganic and Medicinal Chemistry Letters 2008

Protein design by directed evolution
Jäckel, C., Kast, P., and Hilvert, D. Annual review of biophysics 2008 37, 153-173

De novo computational design of retro-aldol enzymes
Jiang, L., Althoff, E. A., Clemente, F. R., Doyle, L., Röthlisberger, D., Zanghellini, A., Gallaher, J. L., Betker, J. L., Tanaka, F., Barbas Iii, C. F., Hilvert, D., Houk, K. N., Stoddard, B. L., and Baker, D.Science 2008 319, 1387-1391

On the relationship between folding and chemical landscapes in enzyme catalysis 
Roca, M., Messer, B., Hilvert, D., and Warshel, A. Proc. Natl. Acad. Sci. U. S. A. 2008 105, 13877-13882

Kinetics and Thermodynamics of Ligand Binding to a Molten Globular Enzyme and Its Native Counterpart
Vamvaca, K., Jelesarov, I., and Hilvert, D. J. Mol. Biol. 2008 382, 971-977

Closely related antibody receptors exploit fundamentally different strategies for steroid recognition
Verdino, P., Aldag, C., Hilvert, D., and Wilson, I. A. Proc. Natl. Acad. Sci. U. S. A. 2008 105, 11725-11730

Catalysis of oxidative protein folding by small-molecule diselenides. 
Beld, J., K. J. Woycechowsky, et al. Biochemistry 2008 47(27): 6985-6987.

Selenoglutaredoxin as a glutathione peroxidase mimic. 
Casi, G., G. Roelfes, et al. ChemBioChem 2008 9(10): 1623-1631.

Conformational isomerism can limit antibody catalysis. 
Debler, E. W., R. Muller, et al. Journal of Biological Chemistry 2008 283(24): 16554-16560.

Switching antibody specificity through minimal mutation. 
Piatesi, A., C. Aldag, et al. Journal of Molecular Biology 2008 377(4): 993-1001.

The putative Diels-Alderase macrophomate synthase is an efficient aldolase. 
Serafimov, J. M., D. Gillingham, et al. Journal of the American Chemical Society 2008 130(25): 7798.

 

2007

 

Reinvestigation of a selenopeptide with purportedly high glutathione peroxidase activity 
Casi, G. & Hilvert, D. Journal of Biological Chemistry 2007 282 (42): 30518-30522

Structure and dynamics of a molten globular enzyme. 
Pervushin, K., K. Vamvaca, et al. Nature Structural & Molecular Biology 2007 14: 1202-1206.

Metabolic engineering of a genetic selection system with tunable stringency 
Kleeb, A.C., Edalat, M.H., Gamper, M., Haugstetter, J., Giger, L., Neuenschwander, M., Kast, P., Hilvert, D. Proceedings of the National Academy of Sciences of the United States of America 2007 104 (35), pp. 13907-13912

A simple selection strategy for evolving highly efficient enzymes
Neuenschwander, M., Butz, M., Heintz, C., Kast, P., Hilvert, D. Nature Biotechnology 2007 25 (10), pp. 1145-1147

Preparation of the β3-homoselenocysteine derivatives Fmoc-β3hSec(PMB)-OH and Boc-β3hSec(PMB)-OH for solution and solid-phase-peptide synthesis and selenoligation
Flögel, O., Casi, G., Hilvert, D., Seebach, D. Helvetica Chimica Acta 2007 90 (9), pp. 1651-1666

Trapping and structural elucidation of an intermediate in the macrophomate synthase reaction pathway
Serafimov, J.M., Westfeld, T., Meier, B.H., Hilvert, D.Journal of the American Chemical Society 2007 129 (31), pp. 9580-9581,

Minimalist active-site redesign: Teaching old enzymes new tricks
Toscano, M.D., Woycechowsky, K.J., Hilvert, D., Angewandte Chemie - International Edition 2007 46 (18), pp. 3212-3236

Enhancing activity and controlling stereoselectivity in a designed PLP-dependent aldolase
Toscano, M.D., Müller, M.M., Hilvert, D., Angewandte Chemie - International Edition 2007 46 (24), pp. 4468-4470

Selenoglutathione: Efficient oxidative protein folding by a diselenide,
Beld, J., Woycechowsky, K.J., Hilvert, D., Biochemistry 2007 46 (18), pp. 5382-5390

Active site mutagenesis of the putative Diels-Alderase macrophomate synthase,
Serafimov, J.M., Lehmann, H.C., Oikawa, H., Hilvert, D., Chemical Communications 2007 (17), pp. 1701-1703

Relative tolerance of mesostable and thermostable protein homologs to extensive mutation, 
Besenmatter, W., Kast, P., Hilvert, D., Proteins: Structure, Function and Genetics 2007 66 (2), pp. 500-506

Synthesis and application of an azobenzene amino acid as a light-switchable turn element in polypeptides, 
Aemissegger, A., Hilvert, D., Nature Protocols 2007 2 (1), pp. 161-167

Bifunctional catalysis of proton transfer at an antibody active site
Müller, R., Debler, E.W., Steinmann, M., Seebeck, F.P., Wilson, I.A., Hilvert, D., Journal of the American Chemical Society 2007 129 (3), pp. 460-461

Novel enzymes through design and evolution
Woycechowsky, K.J., Vamvaca, K., Hilvert, D., Advances in Enzymology and Related Areas of Molecular Biology 2007 75, pp. 241-294, 2007

A monofunctional and thermostable prephenate dehydratase from the archaeon Methanocaldococcus jannaschii (vol 45, pg 14101, 2006).
Kleeb, A. C.; Kast, P.; Hilvert, D.,Biochemistry 2007, 46, (9), 2552-2552.

 

 

2005/2006  

 

Stereoselectivity and expanded substrate scope of an engineered PLP-dependent aldolase
Seebeck, F. P.; Guainazzi, A.; Amoreira, C.; Baldridge, K. K.; Hilvert, D.Angewandte Chemie-International Edition 2006, 45, 6824

A photoswitchable miniprotein based on the sequence of avian pancreatic
Jurt, S.; Aemissegger, A.; Guntert, P.; Zerbe, O.; Hilvert, D.Angewandte Chemie-International Edition 2006, 45, 6297

Tunnel plasticity and quaternary structural integrity of a pentameric protein ring. 
K. J. Woycechowsky, F. P. Seebeck, D. Hilvert, Protein Science 2006, 15, 1106.

A simple tagging system for protein encapsulation. 
F. P. Seebeck, K. J. Woycechowsky, W. Zhuang, J. P. Rabe, D. Hilvert, Journal Of The American Chemical Society 2006, 128, 4516.

Experimental and computational investigation of the uncatalyzed rearrangement and elimination reactions of isochorismate. 
M. S. DeClue, K. K. Baldridge, P. Kast, D. Hilvert, Journal Of The American Chemical Society 2006, 128, 2043.

An active enzyme constructed from a 9-amino acid alphabet. 
K. U. Walter, K. Vamvaca, D. Hilvert, Journal Of Biological Chemistry 2005, 280, 37742.

 

Isochorismate pyruvate lyase: A pericyclic reaction mechanism? 
M. S. DeClue, K. K. Baldridge, D. E. Kunzler, P. Kast, D. Hilvert, Journal Of The American Chemical Society 2005, 127, 15002.

Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection. 
D. Kunzler, S. Sasso, M. Gamper, D. Hilvert, P. Kast, Journal Of Biological Chemistry 2005, 280, 32827.

Isotope effects on the enzymatic and nonenzymatic reactions of chorismate. 
S. K. Wright, M. S. DeClue, A. Mandal, L. Lee, O. Wiest, W. W. Cleland, D. Hilvert, Journal Of The American Chemical Society 2005, 127, 12957.

Conformational diversity and catalysis. 
D. Hilvert, Biopolymers 200580, 492.

Simultaneous optimization of enzyme activity and quaternary structure by directed evolution. 
K. Vamvaca, M. Butz, K. U. Walter, S. V. Taylor, D. Hilvert, Protein Science 2005, 14, 2103.

Conformational diversity and catalysis. 
D. Hilvert, Faseb Journal 200519, A249.

Investigation of ligand binding and protein dynamics in Bacillus subtilis chorismate mutase by transverse relaxation optimized spectroscopy-nuclear magnetic resonance. 
A. Eletsky, A. Kienhofer, D. Hilvert, K. Pervushin, Biochemistry 2005, 44, 6788.

Strategy for chromosomal gene targeting in RecA-deficient Escherichia coli strains. 
M. Gamper, P. Kast, Biotechniques 2005, 38, 405.

Structural origins of efficient proton abstraction from carbon by a catalytic antibody.
Debler, E.W.; Ito, S.; Seebeck, F.P.; Heine, A.; Hilvert, D.; Wilson, I.A. Proc. Natl. Acad. Sci. USA, 2005, 102, 4984-4989.

Use of molecular dynamics in the design and structure determination of a photoinducible beta-hairpin.
Krautler, V; Aemissegger, A.; Hunenberger, P.H.; Hilvert, D.; Hansson, T.; van Gunsteren, W.F. J. Am. Chem. Soc., 2005, 127, 4935-4942.

A photoinducible beta-hairpin.
Aemissegger, A.; Krautler, V.; van Gunsteren, W.F.; Hilvert, D. J. Am. Chem. Soc., 2005, 127, 2929-2936.

Characterization of the secreted chorismate mutase from the pathogen Mycobacterium tuberculosis.
Sasso, S.; Ramakrishnan, C.; Gamper, M.; Hilvert, D.; Kast, P. FEBS J., 2005, 272, 375-389.

Positional ordering of reacting groups contributes significantly to the efficiency of proton transfer at an antibody active site.
Seebeck, F.P; Hilvert, D. J. Am. Chem. Soc., 2005, 127, 1307-1312.

2003/2004  

New enzymes from combinatorial library modules
Besenmatter, W.; Kast, P.; Hilvert, D. Methods Enzymol., 2004, 388, 91-102.

An enzymatic molten globule: Efficient coupling of folding and catalysis
Vamvaca, K.; Vogeli, B.; Kast, P.; Pervushin, K.; Hilvert, D. Proc. Natl. Acad. Sci. USA, 2004, 101, 12860-12864.

Nonspecific medium effects versus specific group positioning in the antibody and albumin catalysis of the base-promoted ring-opening reactions of benzisoxazoles
Hu, Y.F.; Houk, K.N.; Kikuchi, K.; Hotta, K.; Hilvert, D. J. Am. Chem. Soc., 2004, 126, 8197-8205.

Deciphering enzymes - Genetic selection as a probe of structure and mechanism.
Woycechowsky, K.; Hilvert, D. Eur. J. Biochem., 2004, 271, 1630-1637.

Immunological optimization of a generic hydrophobic packet for high affinity hapten binding and Diels-Alder activity.
Piatesi, A.; Hilvert, D. ChemBioChem, 2004, 5, 460-466.

Quantitative evaluation of noncovalent chorismate mutase-inhibitor binding by ESI-MS.
Wendt, S.; McCombie, G.; Daniel, J.; Kienhöfer, A.; Hilvert, D.; Zenobi, R. J. Amer. Soc. Mass. Spectrom., 2003, 14, 1470-1476.

Convergent protein synthesis.
Casi, G.; Hilvert, D. Curr. Opin. Struct. Biol., 2003, 13, 589-594.

Conversion of a PLP-dependent racemase into an aldolase by a single active site mutation.
Seebeck, F.; Hilvert, D. J. Am. Chem. Soc., 2003, 125, 10158-10159.

Incorporation of selenomethionine into proteins through selenohomocysteine-mediated ligation.
Roelfes, G.; Hilvert, D. Angew. Chem. Int. Ed., 2003, 42, 2275-2277.

Promiscuity in antibody catalysis: Esterolytic activity of the decarboxylase 21D8.
Backes, A.; Hotta, K.; Hilvert, D. Helv. Chim. Acta, 2003, 86, 1167-1174.

Charge optimization increases the potency and selectivity of a chorismate mutase inhibitor.
Mandal, A.; Hilvert, D. J. Am. Chem. Soc., 2003, 125, 5598-5599.

Selective stabilization of the chorismate mutase transition state by a positively charged hydrogen bond donor.
Kienhöfer, A.; Kast, P.; Hilvert, D. J. Am. Chem. Soc., 2003, 125, 3206-3207.

Catalysis on the coastline: Theozyme, molecular dynamics, and free energy perturbation analysis of antibody 21D8 catalysis of the decarboxylation of 5-nitro-3-carboxybenzisoxazole.
Ujaque, G.; Tantillo, D.J.; Hu, Y.F.; Houk, K.N.; Hotta, K.; Hilvert, D. J. Comput. Chem., 2003, 24, 98-110.

2001/2002  

Steric and electronic effects on an antibody-catalyzed Diels-Alder reaction.
Gozin, Y.; Hilvert, D. Helv. Chim. Acta, 2002, 85, 4328-4336.

Selenocysteine-mediated backbone cyclization of unprotected peptides followed by alkylation, oxidative elimination or reduction of the selenol.
Quaderer, R.; Hilvert, D. Chem. Commun., 2002, 2620-2621.

Direct NMR observation and DFT calculations of a hydrogen bond at the active site of a 44 kDa enzyme.
Eletsky, A.; Heinz, T.; Moreira, O.; Kienhöfer, A.; Hilvert, D.; Pervushin, K. J. Biomol. NMR, 2002, 24, 31-39.

An antibody that reconstitutes the "base-on" form of B12 coenzymes.
Hannak, R.B.; Konrat, R.; Schüler, W.; Kräutler, B.; Auditor, M.T.M.; Hilvert, D. Angew. Chem. Int. Ed., 2002, 41, 3613-3616.

Investigation of the enzymatic and nonenzymatic cope rearrangement of carbaprephenate to carbachorismate.
Aemissegger, A.; Jaun, B.; Hilvert, D. J. Org. Chem., 2002, 67, 6725-6730.

Optimized production of the Diels-Alderase antibody 1E9 as a chimeric Fab.
Piatesi, A.; Hilvert, D. Can. J. Chem., 2002, 80, 657-664.

Synthesis of b3-peptides and mixed a/b3-peptides by thioligation.
Kimmerlin, T.; Seebach, D.; Hilvert, D. Helv. Chim. Acta, 2002, 85, 1812-1826.

Probing ligand recognition in the decarboxylase antibody 21D8: Implications for the catalytic mechanism.
Hotta, K.; Wilson, I. A.; Hilvert, D. Biochemistry, 2002, 41, 772-779.

Improved synthesis of C-terminal peptide thioesters on "safety-catch" resins using LiBr/THF.
Quaderer, R.; Hilvert, D. Org. Letters, 2001, 3, 3181-3184.

Investigating and engineering enzymes by genetic selection.
Taylor, S. V.; Kast, P.; Hilvert, D. Angew. Chem. Int. Ed., 2001, 40, 3310-3335.

Fmoc-compatible solid-phase peptide synthesis of long C-terminal peptide thioesters.
Sewing, A.; Hilvert, D. Angew. Chem. Int. Ed., 2001, 40, 3395.

Searching sequence space for protein catalysts.
Taylor, S. V.; Walter, K. U.; Kast, P.; Hilvert, D. Proc. Natl. Acad. Sci. USA, 2001, 98, 10596-10601.

Selenocysteine-mediated native chemical ligation.
Quaderer, R.; Sewing, A.; Hilvert, D. Helv. Chim. Acta, 2001, 84, 1197-1206.

Chemical biology/biological chemistry.
Hilvert, D. Chimia, 2001, 55, 267.

Cross-fertilizing the chemical field.
Hilvert, D. C&E News, 2001, 79, 152.

2000 

Shape complementarity, binding-site dynamics, and transition state stabilization: A theoretical study of Diels-Alder catalysis by antibody 1E9.
Chen J. G.; Deng Q.L.; Wang R. X.; Houk, K. N.; Hilvert, D. ChemBioChem, 2000, 1, 255-261.

A strategically positioned cation is crucial for efficient catalysis by chorismate mutase.
Kast, P.; Grisostomi, C.; Chen, I. A.; Li, S. L.; Krengel, U.; Xue, Y. F.; Hilvert, D. J. Biol. Chem., 2000, 275, 36832-36838.

Probing the role of the C-terminus of Bacillus subtilis chorismate mutase by a novel random protein-termination strategy.
Gamper, M.; Hilvert, D.; Kast, P. Biochemistry, 2000, 39, 14087-14094.

Catalysis of decarboxylation by a preorganized heterogeneous microenvironment: Crystal structures of abzyme 21D8.
Hotta, K.; Lange, H.; Tantillo, D. J.; Houk, K. N.; Hilvert, D.; Wilson, L. A. J. Mol. Biol., 2000, 302, 1213-1225.

Catalysis of 3-carboxy-1,2-benzisoxazole decarboxylation by hydrophobic antibody binding pockets.
Hotta, K.; Kikuchi, K.; Hilvert, D. Helv. Chim. Acta, 2000, 83, 2183-2191.

Critical analysis of antibody catalysis.
Hilvert, D. Annu. Rev. Biochem., 2000, 69, 751-793.

Characterization of proton-transfer catalysis by serum albumins.
Hollfelder, F.; Kirby, A. J.; Tawfik, D. S.; Kikuchi, K.; Hilvert, D. J. Am. Chem. Soc., 2000, 122, 1022-1029.

Facile, Fmoc-compatible solid-phase synthesis of peptide C-terminal thioesters.
Swinnen, D.; Hilvert, D. Org. Lett., 2000, 2, 2439-2442.

Molecular dynamics simulations highlight mobile regions in proteins: A novel suggestion for converting a murine Vh domain into a more traceable species.
Voordijk, S.; Hansson, T.; Hilvert, D.; van Gunsteren, W. F. J. Mol. Biol., 2000, 300, 963-973.

Catalysis of decarboxylation by a preorganized heterogeneous microenvironment: crystal structures of abzyme 21D8.
Hotta, K.; Lange, H.; Tantillo, D.; Houk, K. N.; Hilvert, D.; Wilson, I. A. J. Mol. Biol., 2000, 302, 1213-1225.

Catalysis of 3-carboxy-1,2-benzisoxazole decarboxylation by hydrophobic antibody binding pockets.
Hotta, K.; Kikuchi, K.; Hilvert, D. Helv. Chim. Acta, 2000, 83, 2183-2191.

Shape complementarity, binding-site dynamics, and transition state stabilization: A theoretical study of Diels-Alder catalysis by antibody 1E9.
Chen, J.; Deng, Q.; Wang, R.; Houk, K. N.; Hilvert, D. ChemBioChem, 2000, 1, 255-261.

A strategically positioned cation is crucial for efficient catalysis by chorismate mutase.
Kast, P.; Grisostomi, C.; Chen, I. A.; Li, S.; Krengel, U.; Xue, Y.; Hilvert, D. J. Biol. Chem., 2000, 275, 36832-36838.

Probing the role of the C-terminus of Bacillus subtilis chorismate mutase by a novel random protein-termination strategy.
Gamper, M.; Hilvert, D.; Kast, P. Biochemistry, 2000, 39, 14087-14094.

 
 
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28.06.2017
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