2010-2016

Main content

2016

Site-Specific Polymer Conjugation Stabilizes Therapeutic Enzymes in the Gastrointestinal Tract Schulz, J.D., Patt, M., Basler, S., Kries, H., Hilvert, D. Gauthier, M.A., Leroux, J.C. Adv. Mater., 2016, 28, 1455–1460

Chiral Recognition in Amyloid Fiber Growth Torbeev, V., Grogg, M., Ruiz, J., Boehringer, R., Schirer, A., Hellwig, P., Jeschke, G., Hilvert, D. 

A Chemically Programmed Proximal Ligand Enhances the Catalytic Properties of a Heme Enzyme. Green, A.P., Hayashi, T., Hilvert,  D. J. Am. Chem. Soc., 2016, 138, 11344-11352

Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase
Obexer, R., Godina, A., Garrabou, X., Mittl, P. R. E., Baker, D., Griffiths, A. D., Hilvert, D., Nature Chem. 2016, doi:10.1038/nchem.2596

Bottom-up Construction of a Primordial Carboxysome Mimic. Mantri, S., Frey, R., Rocca, M., Hilvert,  D. J. Am. Chem. Soc., 2016, 138, 10072-10075

Protein Nanocontainers from Nonviral Origin: Testing the Mechanics of Artificial and Natural Protein Cages by AFM. Heinze, K., Sasaki, E., King, N.P., Baker, D., Hilvert, D., Wuite, G.J.L., Roos, W.H. J. Phys. Chem. B, 2016, 120, 5945-5952

Upregulation of an Artificial Zymogen by Proteolysis.
Liu, Z., Lebrun, V., Kitanosono, T., Mallin, H., Kohler, V., Haussinger, D., Hilvert, D., Kobayashi, S., Ward, T. R., Angew. Chemie 2016 doi: 10.1002/anie.201605010

Enzyme-Mediated Polymerization inside Engineered Protein Cages. Hayashi, T., Frey, R., Hilvert,  D. Chem. Commun., 2016, 52, 10423-10426

DNA Nanoparticles for Improved Protein Synthesis In Vitro.
Galinis, R., Stonyte, G., Kiseliovas, V., Zilionis, R., Studer, S., Hilvert, D., Janulaitis, A., Mazutis, L., Angew. Chemie 2016 doi: 10.1002/anie.201511809

A Chemically Programmed Proximal Ligand Enhances the Catalytic Properties of a Heme Enzyme.
Green, A. P., Hayashi, T., Mittl, P. R. E., Hilvert, D., JACS 2016, doi:10.1021/jacs.6b07029

Nearer to nature: design and optimization of artificial enzymes.
Hilvert, D., The FASEB Journal 2016

Evaluation of the Ser-His Dipeptide, a Putative Catalyst of Amide and Ester Hydrolysis. MacDonald, M.J., Lavis,  L.D., Hilvert,  D., Gellman, S.H., Org. Lett., 2016, 18, 3518-3521

Self-Assembly of Proteinaceous Multishell Structures Mediated by a Supercharged Protein.
Sasaki, E., Hilvert, D., J. Phys. Chem. 2016, doi:10.1021/acs.jpcb.6b02068

Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting.
Obexer, R., Pott, M., Zeymer, C., Griffiths, A. D., Hilvert, D., Protein Engineering Design & Selection. 2016, doi:10.1093/protein/gzw032

Fast Knoevenagel condensations catalyzed by an artificial Schiff-base forming enzyme.
Garrabou, X., Wicky, B. I. M., Hilvert, D., JACS 2016 doi: 10.1021/jacs.6b00816

2015

Diffusion-Limited Cargo Loading of an Engineered Protein Container. Zschoche, R., Hilvert, D., JACS 2015 doi: 10.1021/jacs.5b10588

Quantitative Packaging of Active Enzymes into a Protein Cage.
Azuma, Y., Zschoche, R., Tinzl, M., Hilvert, D., Angew. Chemie 2015 doi: 10.1002/anie.201508414

A subdomain swap strategy for reengineering nonribosomal peptides.
Kries, H., Niquille, D., Hilvert, D., Chemistry and Biology 2015 doi: 10.1016/j.chembiol.2015.04.015

A Promiscuous De Novo Retro-Aldolase Catalyzes Asymmetric Michael Additions via Schiff Base Intermediates.
Garrabou, X., Beck, T., Hilvert, D., Angew. Chem. 2015, DOI: 10.1002/anie.201500217

Kinetic Consequences of Introducing a Proximal Selenocysteine Ligand into Cytochrome P450cam.
Vandemeulebroucke, A., Aldag, C., Stiebritz, M. T., Reiher, M., Hilvert, D., Biochemistry 2015, DOI: 10.1021/acs.biochem.5b00939

Comparative laboratory evolution of ordered and disordered enzymes. Schulenburg, C., Stark, Y., Künzle, M., Hilvert, D., J Biol Chem. 2015, 290(15):9310-20. doi: 10.1074/jbc.M115.638080.

Substitution of proline32 by a-methylproline preorganizes ß2-microglobulin for oligomerization but not for aggregation into amyloids.Torbeev, V., Ebert, M.O., Dolenc, J., Hilvert, D., J Am Chem Soc. 2015, 137(7):2524-35. doi: 10.1021/ja510109p.

          Harnessing Selenocysteine Reactivity for Oxidative Protein Folding. Metanis, N., Hilvert, D., Chem. Sci. 2015, 6, 322-325

2014

Construction of Matryoshka-Type Structures from Supercharged Protein Nanocages
Beck, T., Tetter, S., Künzle, M., Hilvert, D., Angew. Chem. 2014, DOI: 10.1002/ange.201408677

Reprogramming Nonribosomal Peptide Synthetases for “Clickable” Amino Acids
Kries, H., Wachtel, R., Pabst, A., Wanner, B., Niquille, D., Hilvert, D., Angew. Chem. VIP 2014, 53, 1 – 5

Impact of scaffold rigidity on the design and evolution of an artificial Diels-Alderase
Preiswerk, N., Beck, T., Schulz, J.D., Milovnik, P., Mayer, C., Siegel, J. B., Baker, D. and Hilvert, D., PNAS 2014, doi: 10.1073/pnas.1401073111

Building Proficient Enzymes with Foldamer Prostheses
Mayer, C., Müller, M. M., Gellman, S. H., Hilvert, D., Angew. Chem. 2014, 126, 1 – 5

2013

Both the cis-trans equilibrium and isomerization dynamics of a single proline amide modulate ß2-microglobulin amyloid assembly
Torbeev, V. Y., Hilvert, D., Proc. Natl. Acad. Sci. 2013

Precision is essential for efficient catalysis in an evolved Kemp eliminase
Blomberg, R., Kries, H., Pinkas, D.M., Mittl, P.R., Grütter, M.G., Privett, H.K., Mayo, S.L., Hilvert, D., Nature 2013

Design of Protein Catalysts
Hilvert, D., Annual Reviews of Biochemistry 2013, 82 (1), 447-470

Evolution of a designed retro-aldolase leads to complete active site remodeling
Giger, L., Caner, S., Obexer, R., Kast, P., Baker, D., Ban, N., and Hilvert, D., Nature Chemical Biology 2013, 494-498

A Genetically Encodable Ligand for Transfer Hydrogenation
Mayer, C., Hilvert, D., European Journal of Organic Chemistry 2013, 16, 3427-3431

Affinity maturation of a computationally designed binding protein affords a functional but disordered polypeptide
Butz, M., Kast, P., Hilvert, D., Journal of Structural Biology 2013, in press

Protein Conformational Disorder and Enzyme Catalysis
Schulenburg, C., Hilvert, D., Topics in Current Chemistry 2013

De novo enzymes by computational design
Kries, H., Blomberg, R., Hilvert, D., Curr. Op. Chem. Biol., 2013, 17, 221-228

Directed Evolution of a Primordial Enzyme Provides Insights into the Development of the Genetic Code
Müller, M. M., Allison, J. R., Hongdilokkul, N., Gaillon, L., Kast, P., van Gunsteren, W. F., Marliere, P., Hilvert, D., PLOS Genetics 2013, 9, e1003187

2012

De novo Chemoenzymatic Synthesis of Sialic Acid
Stallforth, P., Matthies, S., Adibekian, A., Gillingham, D. G., Hilvert, D., Seeberger, P. H., Chem. Comm. 2012, 48, 11987-11989

cis-trans Peptide-Bond Isomerization in alpha-Methylproline Derivatives
Torbeev, V.Y., Fumi E., Ebert, M.O., Schweizer, W. B., Hilvert, D., Helvetica Chimica Acta 2012, 95, 2411-2420

Computational Design of Catalytic Dyads and Oxyanion Holes for Ester Hydrolysis
Richter, F., Blomberg, R., Khare, S. D., Kiss, G., Kuzin, A. P., Smith, A. J. T., Gallaher, J., Pianowski, Z., Helgeson, R. C., Grjasnow, A., Xiao, R., Seetharaman, J., Su, M., Vorobiev, S., Lew, S., Forouhar, F., Kornhaber, G. J., Hunt, J. F., Montelione, G. T., Tong, L., Houk, K. N., Hilvert, D., Baker, D., JACS, 2012, 39, 16197-16206

A novel genetic selection system for PLP-dependent threonine aldolases 
Giger, L., Toscano, M. D., Bouzon, M., Marliere, P., Hilvert, D., Tetrahedron, 2012, 68, 7549-7557

Strategic Use of Non-native Diselenide Bridges to Steer Oxidative Protein Folding
Metanis, N., Hilvert, D., Angew. Chem., Int. Ed. Engl., 2012

Harnessing Protein Symmetry for Enzyme Design
Wörsdörfer, B., Henning, L. M., Obexer, R., Hilvert, D., ACS Catalysis, 2012

Iterative approach to computational enzyme design
Privett, H. K., Kiss, G., Lee, T. M., Blomberg, R., Chica, R. A., Thomas, M. L., Hilvert, D., Houk, K. N., Mayo, S. L., PNAS, 2012

Structural Analyses of Covalent Enzyme-Substrate Analog Complexes Reveal Strengths and Limitations of De Novo Enzyme Design
Wang, L., Althoff, E. A., Bolduc, J., Jiang, L., Moody, J., Lassila, J. K., Giger, L., Hilvert, D., Stoddard, B., Baker, D., J. Mol. Biol., 2012, 3, 615-625

Efficient in Vitro Encapsulation of Protein Cargo by an Engineered Protein Container
Wörsdörfer, B.,Pianowski, Z., and Hilvert, D., JACS, 2012, 909-911

2011

The Chemistry of Selenocysteine
Metanis, N., Beld, J., and Hilvert, D., Wiley, Patai's Chemistry of Functional Groups, 2011

Selenoglutathione-Mediated Rescue of Kinetically Trapped Intermediates in Oxidative Protein Folding
Metanis, N., Foletti, C., Beld, J., and Hilvert, D., Israel Journal of Chemistry 2011,51, 953-959

Tailor-Made Peptide Synthetases (Preview)
Kries, H., and Hilvert, D., Chem. Biol. 2011,18, 1206-1207

An N-Terminal Protein Degradation Tag Enables Robust Selection of Highly Active Enzymes
Butz, M., Neuenschwander, M., Kast, P., and Hilvert, D., Biochemistry 2011, 50, 8594-8602

An Artificial Metalloenzyme for Olefin Metathesis
Mayer, C., Gillingham D. G., Ward, T. R., and Hilvert, D., Chem. Comm. 2011,47, 12068-12070

Photolyase-like Repair of Psoralen-Crosslinked Nucleic Acids
Stafforst, T. and Hilvert, D., Angew. Chem., Int. Ed. Engl. 2011, 50, 9483-9486

Directed Evolution of a Protein Container
Wörsdörfer, B., Woycechowsky, K. J. and Hilvert, D., Science 2011, 331(6017), 589-592 (free access)

2010

Diselenides as universal oxidative folding catalysts of diverse proteins
Beld, J., Woycechowsky, K. J., Hilvert, D., Journal of Biotechnology 2010, 150, 481-489

Modulating PNA/DNA Hybridization by Light
Stafforst, T. and Hilvert, D., Angew. Chem., Int. Ed. Engl. 2010, 49, 9998-1001

Biocatalysts by Evolution
Jäckel, C. and Hilvert, D., Curr. Opin. Biotechnol. 2010, 21, 1-7

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction
Siegel, J. B., Zanghellini, A., Lovick, H. M., Kiss, G., Lambert, A. R., St.Clair, J. L., Gallaher, J. L., Hilvert, D., Gelb, M. H., Stoddard, B. L., Houk, K. N., Michael, F. E., Baker, D. , Science 2010, 329, 309-313

Consensus Protein Design without Phylogenetic Bias
Jäckel, C., Bloom, J. D., Kast, P., Arnold, F. H., Hilvert, D., Journal of Molecular Biology 2010, 541-546

Design, Selection, and Characterization of a Split Chorismate Mutase
Müller, M. M., Kries, H., Csuhai, E., Kast, P., Hilvert, D., Protein Science 2010, 1000-1010

C-13 isotope effect on the reaction catalyzed by prephenate dehydratase
Van Fleet, J., Kleeb, A., Kast, P., Hilvert, D., Cleland, W. W., Biochimica et Biophysica Acta - Proteins and Proteomics 2010, 752-754

Small-Molecule Diselenides Catalyze Oxidative Protein Folding in Vivo
Beld, J., Woycechowsky, K. J., Hilvert, D., ACS Chemical Biology 2010,177-182

Chemoenzymatic synthesis of differentially protected 3-deoxysugars 
Gillingham, D. G., Stallforth, P., Adibekian, A., Seeberger P. H. and Hilvert, D., Nature Chemistry 2010, 102-105      

 
 
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